Area of research
Surgery · Cancer Research
Research interest
Research topics from publications: Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease; LncRNA AFAP1-AS Functions as a Competing Endogenous RNA to Regulate RAP1B Expression by sponging miR-181a in the HSCR; Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p in Hirschsprung's disease. Representative work: // Lei Peng 1, 3, * , Guanglin Chen 1, 3, * , Zhongxian Zhu 1, 3, * , Ziyang Shen 1, 3 , Chunxia Du 1, 3 , Rujin Zang 1, 3 , Yang Su 1, 3 , Hua Xie 1, 3 , Hongxing Li 1, 3 , Xiaoqun Xu 1, 3 , Yankai Xia 1, 2 , Weibing Tang 1, 3 1 State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing China 2 Key Laboratory of Modern Toxicology, Nanjing Medical University, Ministry of Education, China 3 Department of Pediatric Surgery, Children’s Hospital of Nanjing Medical University, Nanjing China * These authors contributed equally to this work Correspondence to: Weibing Tang, email: twbcn@163.com Keywords: hirschsprung disease, Background: Long noncoding RNAs (lncRNAs) have recently emerged as important regulators in a broad spectrum of cellular processes including development and disease. Despite the known engagement of the AFAP1-AS in several human diseases, its biological function in Hirschsprung disease (HSCR) remains elusive. Methods: We used qRT-PCR to detect the relative expression of AFAP1-AS in 64 HSCR bowel tissues and matched normal intestinal tissues. The effects of AFAP1-AS on cell proliferation, migration, cell cycle, apoptosis and cytoskeletal organization were evaluated using CCK-8, transwell assay, flow cytometer analysis and immunofluorescence, in 293T and SH-SY5Y cell lines, respectively. Moreove