Area of research
Molecular Biology · Genetics
Research interest
Research topics from publications: Predicting the postmortem interval of burial cadavers based on microbial community succession; Predicting human age by detecting DNA methylation status in hair; Identification of coding region SNPs from specific and sensitive mRNA biomarkers for the deconvolution of the semen donor in a body fluid mixture; Estimating the time since deposition (TsD) in saliva stains using temporal changes in microbial markers; A method of identifying the blood contributor in mixture stains through detecting blood‐specific mRNA polymorphism; A new set of DIP‐SNP markers for detection of unbalanced and degraded DNA mixtures; Identification of the vaginal secretion donor in mixture stains using polymorphic cSNPs on mRNA biomarkers; The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR; DIP-microhaplotypes: new markers for detection of unbalanced DNA mixtures; A new set of 20 Multi‐InDel markers for forensic application. Representative work: Age prediction is of great importance for criminal investigation and judicial expertise. DNA methylation status is considered a promising method to infer tissue age by virtue of age-dependent changes on methylation sites. In recent years, forensic scientists have established various models to predict the chronological age of blood, saliva, and semen based on DNA methylation status. However, hair-inferred age has not been studied in the field of forensic science. In this study, we measured the methylation statuses of potential age-related CpG sites by using the multiplex methylation SNaPshot method. A total of 10 CpG sites from the LAG3, SCGN, ELOVL2, KLF14, C1orf132, SLC12A5, GRIA2, and PDE4 In the past decades, messenger RNA (mRNA) biomarkers have been employed to identify the origin of body fluids in forensic medicine. We hypothesized that the polymorphism of mRNA could be applied to identify individuals in mixture samples composed of two body fluids. In this study, we selected five blood-specific mRNA biomarkers of venous blood (SPTB, CD3G, AMICA1, ANK1, and GYPA) that encompass 16 SNPs to identify the mixture contributor(s). Five specific gene markers for menstrual blood, semen, skin, saliva, and vaginal secretions were amplified and typed as body-fluid positive controls. We established the system of multiplex PCR and single base extension (SBE) reaction followed
On the influence of various factors on the recovery efficiency and individual identification capability of exfoliated cells on chopsticks
Identification of the body fluid donor in mixtures through target mRNA cSNP sequencing
mRNA for Body Fluid and Individual Identification
Developmental Validation of a DIP‐InDel/DIP‐STR System for the Detection of Unbalanced DNA Mixtures
Human identification of single hair shaft using a mass spectrometry mRNA typing system
Epigenetic Age Estimation by Detecting DNA Methylation Status in Buccal Swabs
A preliminary study characterizing temporal changes in soil bacterial communities after dismembered bones were buried
Development of a novel panel for blood identification based on blood-specific CpG-linked SNP markers
A human identification system for hair shaft using RNA polymorphism
Estimating the time since deposition (TsD) in saliva stains using temporal changes in microbial markers
Identification of the vaginal secretion donor in mixture stains using polymorphic cSNPs on mRNA biomarkers
A new set of 20 Multi‐InDel markers for forensic application
A recombinase polymerase amplification (RPA) combined with strip visualization method for RNA-based presumptive tests of saliva and vaginal secretion
Typing of semen-containing mixtures using ARMS-based semen-specific CpG-InDel/STR markers
Predicting the postmortem interval of burial cadavers based on microbial community succession
Predicting human age by detecting DNA methylation status in hair
Identification of coding region SNPs from specific and sensitive mRNA biomarkers for the deconvolution of the semen donor in a body fluid mixture
Development of a multiplex methylation-sensitive restriction enzyme-based SNP typing system for deconvolution of semen-containing mixtures
A method of identifying the blood contributor in mixture stains through detecting blood‐specific mRNA polymorphism
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR
DIP-microhaplotypes: new markers for detection of unbalanced DNA mixtures
DNA typing from skeletal remains: a comparison between capillary electrophoresis and massively parallel sequencing platforms
A new set of DIP‐SNP markers for detection of unbalanced and degraded DNA mixtures
Multiple methods used for type detection of uniparental disomy in paternity testing
Genetic polymorphisms and mutation rates of 16 X-STRs in a Han Chinese population of Beijing and application examples in second-degree kinship cases
Involvement of sphingosine-1-phosphate receptors 2/3 in IR-induced sudden cardiac death
A SNaPshot assay for detection of 45 mutations in the SCN5A gene in the Chinese Han Population
A mixture detection method based on separate amplification using primer specific alleles of INDELs-a study based on two person's DNA mixture