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Scot A. Wolfe

Washington University in St. Louis · US
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Area of research
Molecular Biology · Genetics
Research interest
Research interests include CRISPR and Genetic Engineering, Genomics and Chromatin Dynamics, Advanced biosensing and bioanalysis techniques, and RNA and protein synthesis mechanisms.
h-index
61
citations
13,981
works
180
NIH funding
primary concept
Biology
email

Recent publications

Prime assembly with linear DNA donors enables large genomic insertions.
2026cited by 0position: contributordoi
Adissp activates insulin-independent glucose disposal and energy expenditure in white fat to treat diabetes and cardiometabolic disease.
2026cited by 0position: contributordoi
SORT LNPs encapsulating Cas9 mRNA achieve efficient editing in skeletal muscle in a dystrophic mouse model
Molecular Therapy 2026cited by 0position: contributordoi
Systemic delivery of CRISPR-Cas9 nickase suppresses oncogene amplified cancer progression
2026cited by 0position: contributordoi
Increasing intracellular dNTP levels improves prime editing efficiency.
2025cited by 16position: contributordoi
Selective targeting of genome amplifications and repeat elements by CRISPR-Cas9 nickases to promote cancer cell death.
2025cited by 4position: contributordoi
Direct delivery of Cas-embedded cytosine base editors as ribonucleoprotein complexes for efficient and accurate editing of clinically relevant targets.
2025cited by 4position: contributordoi
Efficient Cas9 nuclease-based editing in skeletal muscle via lipid nanoparticle delivery
2025cited by 2position: contributordoi
Self-delivering, chemically modified CRISPR RNAs for AAV co-delivery and genome editing in vivo.
2024cited by 11position: contributordoi
Ex vivo culture resting time impacts transplantation outcomes of genome-edited human hematopoietic stem and progenitor cells in xenograft mouse models
Cytotherapy 2024cited by 4position: contributordoi
Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets
2024cited by 1position: contributordoi
PRC1.6 localizes on chromatin with the human silencing hub (HUSH) complex for promoter-specific silencing
2024cited by 0position: contributordoi
Human genetic diversity alters off-target outcomes of therapeutic gene editing.
2023cited by 98position: contributordoi
Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag
Nature Methods 2023cited by 72position: lastdoi
Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag.
2023cited by 59position: contributordoi
Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency.
2023cited by 51position: contributordoi
Self-delivering, chemically modified CRISPR RNAs for AAV co-delivery and genome editing <i>in vivo</i>
Nucleic Acids Research 2023cited by 16position: middledoi
Runx1-R188Q germ line mutation induces inflammation and predisposition to hematologic malignancies in mice.
2023cited by 8position: contributordoi
Gene editing without <i>ex vivo</i> culture evades genotoxicity in human hematopoietic stem cells
2023cited by 7position: contributordoi
Addressing the dNTP bottleneck restricting prime editing activity
2023cited by 7position: contributordoi
Self-delivering CRISPR RNAs for AAV Co-delivery and Genome Editing <i>in vivo</i>
2023cited by 1position: contributordoi
A brown fat-enriched adipokine, ASRA, is a leptin receptor antagonist that stimulates appetite
2023cited by 0position: contributordoi
LONP-1 and ATFS-1 sustain deleterious heteroplasmy by promoting mtDNA replication in dysfunctional mitochondria.
2022cited by 87position: contributordoi
A flexible split prime editor using truncated reverse transcriptase improves dual-AAV delivery in mouse liver.
2022cited by 84position: contributordoi
Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors
The CRISPR Journal 2022cited by 51position: lastdoi
Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors.
2022cited by 45position: contributordoi
Genome-wide detection of CRISPR editing in vivo using GUIDE-tag.
2022cited by 42position: contributordoi
Optimization of NLS Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells
2022cited by 0position: contributordoi
Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice.
2021cited by 226position: contributordoi
The NIH Somatic Cell Genome Editing program
Nature 2021cited by 129position: middledoi

Grants

No grants ingested yet.

Frequent collaborators

· 42 papers (2019–2026)Erik J. Sontheimer · Inspire17 papers (2019–2026)Lihua Julie Zhu · Wenzhou Medical University8 papers (2016–2023)Daniel E. Bauer · Kingston University8 papers (2019–2024)Lihua Julie Zhu · University of Chinese Academy of Sciences8 papers (2019–2026)Wen Xue · Chengdu University of Traditional Chinese Medicine8 papers (2020–2026)Pengpeng Liu · University of Massachusetts Chan Medical School7 papers (2019–2026)Raed Ibraheim · Bard College6 papers (2019–2022)Yuxuan Wu · Wenzhou Medical University5 papers (2019–2020)Kevin Luk · University of Massachusetts Chan Medical School5 papers (2019–2022)Jeremy Luban · Harvard University Press5 papers (2021–2025)Guangping Gao · University of Massachusetts Chan Medical School5 papers (2020–2023)Shun-Qing Liang · University Hospital of Bern5 papers (2020–2022)Erik J. Sontheimer · University of Massachusetts Chan Medical School4 papers (2018–2023)Wen Xue · Tianjin University of Science and Technology4 papers (2016–2023)Sneha Suresh · Massachusetts Institute of Technology4 papers (2016–2023)Daniel E. Bauer · Harvard University4 papers (2019–2020)Qiuming Yao · University of Nebraska–Lincoln4 papers (2019–2020)Jonathan K. Watts · NeuroNexus (United States)4 papers (2022–2025)Emmanouela Tsagkaraki · University of Crete4 papers (2020–2021)
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