← back to search

John M. Asara

Harvard University · US
Area of research
Molecular Biology · Cancer Research
Research interest
The main area of our research involves the use of high resolution mass spectrometry to quantitatively study protein complexes, post-translational modifications, metabolomics and lipidomics in signaling pathways for diseases such as cancer. The goal is to identify new targets for drugs that specifically inhibit the defective pathways that are driving cell growth, proliferation and metastasis. We recently developed a mass spectrometry approach for label-free quantitative proteomics based on the average and total signal values of the peptide LC/MS/MS spectra identified from each protein.
h-index
115
citations
61,199
works
837
NIH funding
primary concept
Chemistry
email

Recent publications

Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle
Molecular Cell 2025cited by 5position: middledoi
Multiomic analysis reveals a key BCAT1 role in mTOR activation by B cell receptor and TLR9
Journal of Clinical Investigation 2025cited by 3position: middledoi
The SLC1A1/EAAT3 dicarboxylic amino acid transporter is an epigenetically dysregulated nutrient carrier that sustains oncogenic metabolic programs
Nature Communications 2025cited by 2position: middledoi
Methylmalonic acid induces metabolic abnormalities and exhaustion in CD8+ T cells to suppress anti-tumor immunity
Oncogene 2024cited by 14position: middledoi
Parallel phosphoproteomics and metabolomics map the global metabolic tyrosine phosphoproteome
bioRxiv (Cold Spring Harbor Laboratory) 2024cited by 1position: middledoi
PRMT1 mediated methylation of cGAS suppresses anti-tumor immunity
Nature Communications 2023cited by 75position: middledoi
A phosphate-sensing organelle regulates phosphate and tissue homeostasis
Nature 2023cited by 42position: middledoi
AMPK-dependent phosphorylation of the GATOR2 component WDR24 suppresses glucose-mediated mTORC1 activation
Nature Metabolism 2023cited by 40position: middledoi
LKB1-Dependent Regulation of TPI1 Creates a Divergent Metabolic Liability between Human and Mouse Lung Adenocarcinoma
Cancer Discovery 2023cited by 38position: middledoi
NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis
Redox Biology 2023cited by 34position: middledoi
De novo pyrimidine synthesis is a targetable vulnerability in IDH mutant glioma
Cancer Cell 2022cited by 128position: middledoi
Altered propionate metabolism contributes to tumour progression and aggressiveness
Nature Metabolism 2022cited by 107position: middledoi
PI3K drives the de novo synthesis of coenzyme A from vitamin B5
Nature 2022cited by 98position: middledoi
SHP-2 and PD-1-SHP-2 signaling regulate myeloid cell differentiation and antitumor responses
Nature Immunology 2022cited by 82position: middledoi
Lysosomal cystine mobilization shapes the response of TORC1 and tissue growth to fasting
Science 2022cited by 74position: middledoi
PARP-inhibition reprograms macrophages toward an anti-tumor phenotype
Cell Reports 2022cited by 55position: middledoi
mTORC1 regulates a lysosome-dependent adaptive shift in intracellular lipid species
Nature Metabolism 2022cited by 51position: middledoi
Suppression of nuclear GSK3 signaling promotes serine/one-carbon metabolism and confers metabolic vulnerability in lung cancer cells
Science Advances 2022cited by 49position: middledoi
Prolonged deprivation of arginine or leucine induces PI3K/Akt-dependent reactivation of mTORC1
Journal of Biological Chemistry 2022cited by 26position: middledoi
Trans-omics analysis of insulin action reveals a cell growth subnetwork which co-regulates anabolic processes
iScience 2022cited by 11position: middledoi
Fatty acid synthesis is required for breast cancer brain metastasis
Nature Cancer 2021cited by 319position: middledoi
GOT1 inhibition promotes pancreatic cancer cell death by ferroptosis
Nature Communications 2021cited by 254position: middledoi
The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals
eLife 2021cited by 240position: middledoi
Proteomics of protein trafficking by in vivo tissue-specific labeling
Nature Communications 2021cited by 113position: middledoi
mTORC1-chaperonin CCT signaling regulates m <sup>6</sup> A RNA methylation to suppress autophagy
Proceedings of the National Academy of Sciences 2021cited by 70position: middledoi
Impaired anaplerosis is a major contributor to glycolysis inhibitor toxicity in glioma
Cancer & Metabolism 2021cited by 24position: middledoi
Targeted deletion of PD-1 in myeloid cells induces antitumor immunity
Science Immunology 2020cited by 438position: middledoi
Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer
Cell Reports 2020cited by 277position: middledoi
Age-induced accumulation of methylmalonic acid promotes tumour progression
Nature 2020cited by 245position: middledoi
Skp2 dictates cell cycle-dependent metabolic oscillation between glycolysis and TCA cycle
Cell Research 2020cited by 113position: middledoi

Grants

MRI: An Ultra High Resolution Mass Spectrometer to Identify Novel Protein Sequences and Modifications from Extinct Organisms Such as Tyrannosaurus Rex
NSF0722702$200,0002007–2009PIRePORTER
SGER: A Method to Sequence Novel Peptides from Unsequenced Taxa: Soft tissue of the 68M Year Old Tyrannosaurus rex
NSF0634136$112,7362006–2008PIRePORTER

Frequent collaborators

Lewis C. Cantley · Meyer (China)29 papers (2012–2024)Min Yuan · Tongji University16 papers (2012–2023)Costas A. Lyssiotis · University of Washington Applied Physics Laboratory12 papers (2012–2018)Norbert Perrimon · Harvard University11 papers (2015–2023)Wenyi Wei · Beth Israel Deaconess Medical Center11 papers (2013–2023)John Blenis · Cornell University9 papers (2013–2022)Pier Paolo Pandolfi · The Ohio State University8 papers (2014–2020)Jianping Guo · Sun Yat-sen University7 papers (2015–2023)Brendan D. Manning · Harvard University7 papers (2012–2022)Pengda Liu · University of North Carolina at Chapel Hill7 papers (2013–2020) · 7 papers (2013–2023)Evan C. Lien · Massachusetts Institute of Technology7 papers (2016–2022)Yanhui Hu · Harvard University7 papers (2015–2023)Wenjian Gan · Indiana University School of Medicine7 papers (2013–2020)Alex Toker · Beth Israel Deaconess Medical Center6 papers (2014–2024)Ana P. Gomes · Moffitt Cancer Center6 papers (2016–2024)Edouard Mullarky · Cornell University6 papers (2013–2022)Gina M. DeNicola · Moffitt Cancer Center5 papers (2013–2023)Christoph W. Turck · Kunming Institute of Zoology5 papers (2016–2018)Jinfang Zhang · Guangzhou University of Chinese Medicine5 papers (2015–2020)