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Shengdar Q. Tsai

St. Jude Children's Research Hospital · US
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Area of research
Molecular Biology · Genetics
Research interest
Research interests include CRISPR and Genetic Engineering, Virus-based gene therapy research, RNA and protein synthesis mechanisms, and Advanced biosensing and bioanalysis techniques.
h-index
50
citations
22,324
works
147
NIH funding
primary concept
email

Recent publications

Monitoring biological effects of somatic cell genome editing
Nature Reviews Genetics 2026cited by 4position: middledoi
Potent and uniform fetal hemoglobin induction via base editing
Nature Genetics 2023cited by 74position: middledoi
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy
Nature Biomedical Engineering 2023cited by 42position: middledoi
Base editing as a genetic treatment for spinal muscular atrophy
bioRxiv (Cold Spring Harbor Laboratory) 2023cited by 17position: middledoi
Base editing of haematopoietic stem cells rescues sickle cell disease in mice
Nature 2021cited by 373position: middledoi
The NIH Somatic Cell Genome Editing program
Nature 2021cited by 129position: middledoi
Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Genome biology 2021cited by 98position: middledoi
Prediction and validation of hematopoietic stem and progenitor cell off-target editing in transplanted rhesus macaques
Molecular Therapy 2021cited by 21position: middledoi
Adenosine Base Editing of γ-Globin Promoters Induces Fetal Hemoglobin and Inhibit Erythroid Sickling
Blood 2020cited by 10position: middledoi
Base Editing Eliminates the Sickle Cell Mutation and Pathology in Hematopoietic Stem Cells Derived Erythroid Cells
Blood 2020cited by 6position: middledoi
Highly efficient therapeutic gene editing of human hematopoietic stem cells
Nature Medicine 2019cited by 514position: middledoi
High levels of AAV vector integration into CRISPR-induced DNA breaks
Nature Communications 2019cited by 424position: middledoi
Zebrafish<i>dscaml1</i>Deficiency Impairs Retinal Patterning and Oculomotor Function
Journal of Neuroscience 2019cited by 21position: middledoi
In vivo CRISPR editing with no detectable genome-wide off-target mutations
Nature 2018cited by 321position: middledoi
CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
Nature Methods 2017cited by 821position: firstdoi
Nodal patterning without Lefty inhibitory feedback is functional but fragile
eLife 2017cited by 67position: middledoi
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
Nature 2016cited by 2,670position: middledoi
Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells
Nature Biotechnology 2016cited by 711position: middledoi
Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases
Nature Reviews Genetics 2016cited by 502position: firstdoi
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
Nature 2015cited by 1,656position: middledoi
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition
Nature Biotechnology 2015cited by 628position: middledoi
Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
Nature Methods 2015cited by 123position: middledoi
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
Nature Biotechnology 2014cited by 2,288position: firstdoi
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
Nature Biotechnology 2014cited by 964position: firstdoi
Toddler: An Embryonic Signal That Promotes Cell Movement via Apelin Receptors
Science 2014cited by 625position: middledoi
Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity
Nature Methods 2014cited by 213position: middledoi
Efficient genome editing in zebrafish using a CRISPR-Cas system
Nature Biotechnology 2013cited by 2,996position: middledoi
Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins
Nature Biotechnology 2013cited by 506position: middledoi
FLASH assembly of TALENs for high-throughput genome editing
Nature Biotechnology 2012cited by 1,195position: middledoi
Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs
Nucleic Acids Research 2012cited by 245position: middledoi

Grants

No grants ingested yet.

Frequent collaborators

J. Keith Joung · Harvard University19 papers (2012–2019)Deepak Reyon · Fidelity Biosciences (United States)9 papers (2012–2019)Nathalie T. Nguyen · University of Rochester Medical Center7 papers (2014–2018)Martin J. Aryee · Harvard University7 papers (2014–2019)Benjamin P. Kleinstiver · Harvard University7 papers (2015–2023)Jeffry D. Sander · Corteva (United States)6 papers (2012–2015) · 5 papers (2019–2023)David R. Liu · Broad Institute5 papers (2014–2021)Ved V. Topkar · Stanford University4 papers (2014–2017)Zongli Zheng · Tung Wah College4 papers (2014–2016)Cyd Khayter · Center for Cancer Research4 papers (2012–2014)Michelle S. Prew · Center for Cancer Research4 papers (2015–2016)James A. Gagnon · University of Utah3 papers (2014–2019)Gregory A. Newby · Elon University3 papers (2020–2021)Randall T. Peterson · University of Utah3 papers (2012–2015)Casey A. Maguire · Baylor University3 papers (2019–2023)Alexander F. Schier · University of Basel3 papers (2014–2019)Jose Malagon-Lopez · Gynuity Health Projects3 papers (2016–2018)Sara P. Garcia · Centro de Investigación Biomédica en Red de Cáncer2 papers (2018–2019)Yu Yao · Nanjing University of Chinese Medicine2 papers (2020–2020)
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