Area of research
Biomedical Engineering · Spectroscopy
Research interest
Research topics from publications: Combined use of [TBA][L-ASP] and hydroxypropyl-β-cyclodextrin as selectors for separation of Cinchona alkaloids by capillary electrophoresis; Simultaneous separation and rapid determination of spironolactone and its metabolite canrenone in different pharmaceutical formulations and urinary matrices by capillary zone electrophoresis; Determination of Cinchona Alkaloids by Capillary Electrophoresis with Novel Complex Formation; Safflower injection against obesity-induced mice podocyte injury by improving insulin resistance through increasing renal INSR and eNOS expression; Separation and determination of cinchona alkaloids by ligand-exchange capillary electrophoresis using a Cu(II)–l-lysine complex as selector. Representative work: The aim of this study was to develop a novel, sensitive, precise, simple, and rapid capillary zone electrophoresis method for the quality control of spironolactone in three different formulation types and a rapid simultaneous determination of the content of spironolactone and canrenone in urine samples using fluocinonide as an internal standard. After optimization of separation conditions, the electrolyte solution was the pH 5.5, 20 mM phosphate buffer containing 4.5 g/L sulfated-β-cyclodextrin, 15 kV of electric filed across the capillary applied at 25°C. A diode array detector was used, and the detection wavelength was 260 nm. Under optimum conditions, good linearity was achieved with corr The formation of a novel complex with cyclodextrin was investigated for the separation of cinchona alkaloids including the diastereomeric pairs of quinine, quinidine, cinchonine, and cinchonidine. The optimized separation of mixed cinchona alkaloids was obtained using 18 mM tris(hydroxymethyl)aminomethane, 3 mM Cu(II), and 20 mM hydroxypropyl-β-cyclodextrin at pH 5.7 with an applied voltage of 20 kV. Under these conditions, the four cinchona alkaloids were completely separated with resolution values between 9.08 and 3.94. The linear dynamic ranges for the analytes were from 10 to 1000 µg/mL with separation in less than 30 minutes. This method was used for the analysis of cinchona bark, quini
Safflower injection against obesity-induced mice podocyte injury by improving insulin resistance through increasing renal INSR and eNOS expression
Separation and determination of cinchona alkaloids by ligand-exchange capillary electrophoresis using a Cu(II)–l-lysine complex as selector
Simultaneous separation and rapid determination of spironolactone and its metabolite canrenone in different pharmaceutical formulations and urinary matrices by capillary zone electrophoresis
Determination of Cinchona Alkaloids by Capillary Electrophoresis with Novel Complex Formation
Combined use of [TBA][L-ASP] and hydroxypropyl-β-cyclodextrin as selectors for separation of Cinchona alkaloids by capillary electrophoresis