Yumei Huo

Area of research

Molecular Biology · Genetics

Research interest

Research topics from publications: Body fluids should be identified before estimating the time since deposition (TsD) in microbiome-based stain analyses for forensics; Rapid and visual detection of specific bacteria for saliva and vaginal fluid identification with the lateral flow dipstick strategy; Evaluation of reverse transcription yield of RNA standards and forensic samples based on droplet digital PCR; Forensic Age Estimation From Blood Samples by Combining DNA Methylation and MicroRNA Markers Using Droplet Digital PCR. Representative work: Identification and the time since deposition (TsD) estimation of body fluid stains from a crime scene could provide valuable information for solving the cases and are always difficult for forensics. Microbial characteristics were considered as a promising biomarker to address the issues. However, changes in the microbiota may damage the specific characteristics of body fluids. Correspondingly, incorrect body fluid identification may result in inaccurate TsD estimation. The mutual influence is not well understood and limited the codetection. In the current study, saliva, semen, vaginal secretion, and menstrual blood samples were exposed to indoor conditions and collected at eight time points ABSTRACT Age estimation is important in criminal investigations and forensic practice, and extensive studies have focused on age determination based on DNA methylation (DNAm) and miRNA markers. Interestingly, it has been reported that combining different types of molecular omics data helps build more accurate predictive models. However, few studies have compared the application of combined DNAm and miRNA data to predict age in the same cohort. In this study, a novel multiplex droplet digital PCR (ddPCR) system that allows for the simultaneous detection of age‐associated DNAm and miRNA markers, including KLF14 , miR‐106b‐5p , and two reference genes ( C‐LESS‐C1 and RNU6B ), was developed. Nex